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Polyplus Transfection

Polyplus-transfection is focused on developing reagents for intracellular delivery of biomolecules. Its expertise includes in vitro and in vivo delivery of genes, oligonucleotides, siRNA and proteins.

With over half its revenues reinvested into R&D, Polyplus-transfection covers all aspects including the physical chemistry, cell biology and pharmacology of carriers. This leads to constant improvement of existing solutions as well as original compounds at the forefront of delivery science.

Country of Origin:  France

Product Range

Transfection of 1. DNA in vitro - jetPRIME™

If you routinely perform transient gene expression studies and/or gene silencing by RNA interference, the new powerful jetPRIME™ is the reagent for you!

Transfection with this versatile reagent requires low quantities of nucleic acid and small amounts of reagent. Therefore, jetPRIME™ is both gentle to cells and economical. A simple and convenient protocol ensures that it’s easy to scale up or scale down.

Features/Benefits:

  • Outstanding DNA transfection efficiency
  • Economical: less DNA and less reagent needed
  • Better cell viability
  • Excellent gene silencing
  • Cotransfection of DNA & siRNA
  • shRNA and miRNA plasmid transfection
  • Convenient protocol

Specifications:

Catalog NumberDescription
114-01jetPRIME kit 0.1 ml of jetPRIME + 5 ml of jetPRIME buffer
114-07jetPRIME kit 0.75 ml of jetPRIME + 40 ml of jetPRIME buffer
114-15jetPRIME kit 1.5 ml of jetPRIME + 2 x (40 ml of jetPRIME buffer)
114-75jetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 55 ml of jetPRIME buffer 10x (conc.)
114-75CjetPRIME kit 5 x (1.5 ml of jetPRIME) + 1 x 120 ml of jetPRIME buffer 5x (conc.)

1.5 ml is sufficient to perform ca.375 transfections in 6-well plates. Bulk quantities are available upon request

Contact us for a trial size of jetPRIME™ DNA & siRNA transfection reagent. Special offer with your first order of jetPRIME™!

For more information on jetPRIME™, click here.

Transfection of 2. siRNA in vitro - INTERFERin™

INTERFERin™ siRNA transfection reagent achieves over 90 % silencing efficiency at 1 nM siRNA in a wide variety of adherent and primary cells with excellent cell viability. Using low siRNA concentrations avoids unwanted toxic and off-target (non-specific) effects observed when transfecting siRNA at higher concentrations, resulting in more robust data.

Features/Benefits:

  • Ready-to-use
  • Compatible with serum and antibiotics
  • Easy standard protocol
  • Gentle mode of action for more robust data and excellent cell viability

Specifications:

CATALOG #DESCRIPTIONNUMBER OF TRANSFECTIONS
409-01INTERFERin™ (0.1ml)50-100 siRNA transfections in 24-well plates (1 nM siRNA concentration)
409-10INTERFERin™ (1ml)500-1000 siRNA transfections in 24-well plates (1 nM siRNA concentration)
409-50INTERFERin™ (5 x 1ml)5000-10000 siRNA transfections in 24-well plates (1 nM siRNA concentration)

1 ml of INTERFERin™ is sufficient to perform 500-1000 transfections in 24-well plates.

For more information on siRNA in vitro - INTERFERin™, click here.

Transfection of 3. DNA for HTS application - jetPEI™

jetPEI™ transfection reagent is particularly well suited for automated or manual HTS (High Throughput Screening) as transfection with jetPEI™ is exceptionally efficient and reproducible. A distinctive linear polyethylenimine (PEI) derivative, jetPEI™ gently facilitates intracellular DNA delivery for high performance.

Three HTS protocols are available depending on the experiment: forward, batch or reverse.

Figure: HEK-293 cells expressing GFP after transfection using jetPEI™.

Features/Benefits:

  • Fast and efficient methods to transfect cells for HTS
  • Compatible with serum and antibiotics
  • Robust jetPEI/DNA complexes
  • Exceptionally reproducible results
  • Well-suited for automated approaches

Specifications:

CATALOG #Amount of reagentAmount of NaCl solution
101-01N0.1 mL5mL
101-10N1 mL50 mL
101-101 mL-
101-40N4 x 1mL4 x 50 mL
101-404 x 1 mL-
101B-010N10 mL2 x 250 mL
101B-01010 mL-

 

One ml of jetPEI™ is sufficient to perform 2 000 transfections in 96-well plates.

For more information on DNA for HTS application - jetPEI™, click here.

Transfection of 4. siRNA for HTS application - INTERFERin™-HTS

NEW! INTERFERin™-HTS is a new generation siRNA transfection reagent especially developed for high throughput screening (HTS) applications providing great silencing efficiency, excellent reproducibility and high cell viability with very low amounts of reagent.

INTERFERin™-HTS is cost-effective, easy to handle, compatible with serum and antibiotics, and comes with reverse and forward protocols for 96- and 384-well plates.

Figure 4. siRNA transfection with INTERFERin™-HTS gives higher silencing efficiency than competitor reagents. HeLa cells were transfected in 96-well plates with 10 nM siRNA duplexes targeting lamin A/C following a reverse transfection protocol according to the manufacturer‘s recommendations. Lamin A/C expression was measured 24 hours after transfection by branched DNA assay and normalized to GAPDH expression.

Features:

  • Great silencing from 10 to 50 nM siRNA
  • Designed for automated procedures
  • Reproducible at all levels
  • Small volume of reagent per well, cost effective

Specifications

Catalog NumberAmount of reagent
410-0020.2 mL
410-0151.5 mL
410-0604 x 1.5 m

1.5 mL of INTERFERin™-HTS is sufficient to transfect 200-300 plates in (96-well)

For more information on INTERFERin™-HTS, click here.

Transfection of 5. Nucleic acid in vivo - in vivo-jetPEI™

Non-viral gene delivery to animals is easy with in vivo-jetPEI™ transfection reagent. Simply mix your DNA (or siRNA) with this reagent and administer to animals for gene expression in multiple tissues.

With in vivo-jetPEI™, you can also deliver genes by different modes into different species based on your needs (even humans in GMP conditions). You'll avoid experimental and regulatory hassles by choosing in vivo-jetPEI™ in place of virus for gene delivery.

This reagent can be made under GMP conditions and customized to target specific cells.

Figure : Non-invasive bioluminescence of a living mouse following systemic delivery of in vivo-jetPEI™/DNA complexes.

Features:

  • Successful delivery of DNA, siRNA and oligonucleotides in vivo
  • Multiple modes of administration in many species
  • RNAi with in vivo-jetPEI™
  • No detectable inflammatory response
  • Currently used in several phase I-II clinical trials
  • Reproducible results

Specifications:

CATALOG #Amount of reagentAmount of glucose solution
201-10G0.1ml10 mL
201-50G0.5 mL2 x 10 mL

0.1ml of in vivo-jetPEI™ is sufficient to perform up to 20 intravenous injections in mouse with 50 µg of DNA.

For more information on in vivo-jetPEI™, click here.

Transfection of 6. DNA for Protein and virus production in synthetic media - Fecturin™

Fecturin™ is the ideal reagent for the production of protein in serum free media. It is formulated to work in a variety of media and cell lines including HEK 293, EBNA and CHO cells giving you the flexibility to produce proteins in your desired host system.

Figure 3. Compaison of protein expression levels after transfection of suspension CHO cells with Fecturin™ and LP2 in two commercially available synthetic media. Cells were seeded in either I-CHO or C-CHO synthetic media and transfected with pCMV-Luc plasmid using either Fecturin™ or LP2 following the manufacturer’s recommendations. Luciferase expression (RLU) was assayed 24h after transfection.

Features:

  • High yields of recombinant protein
  • Specifically developed for transfection of HEK-293 and CHO in synthetic media
  • Free of animal-origin material

Specifications:

CATALOG #Amount of reagentDNA delivered
109-0011mL0.3 to 1 mg

Large volumes are available upon request (10 ml, 50 ml, 100 ml, 1 L).

For more information on Fecturin™, click here.

Transfection of 7. Protein, Antibody and Peptide - PULSin™

PULSin™ is an innovative reagent for the highly efficient transfer of functional proteins to the cytoplasm of living cells. With PULSin™, you can target intracellular proteins in living cells with antibodies without fixation, eliminating the time and effort of fixing cells.

PULSin™ also has advantages over DNA transfection because the amount and timing of exogenous proteins in cells can be controlled. With this precise control even lethal proteins can be studied.

Simple and fast protocol

Features:

  • Effective and robust delivery into the cytoplasm
  • Delivery to a wide variety of cells including primary cells, with up to 98% efficiency
  • Highly efficient transfer
  • Easy to use (Simply add PULSin™ to the protein, incubate, and apply to cells)
  • Easily perform real time function blocking and protein localization studies using antibodies.

Specifications:

Catalog #ReagentNumber of Reactions
501-01PULSin™ (0.1ml)*6 reactions in 6-well plates
501-04PULSin™ (0.4ml)*24 reactions in 6-well plates
501-16PULSin™ (0.4ml)*96 reactions in 6-well plates

0.4 ml of PULSin™ protein delivery reagent is sufficient for 24 reactions in 6-well plates.

For more information on PULSin™, click here.