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As one of the leading life science reagent suppliers in Japan, Nacalai Tesque is also Japan's homegrown brand that offers high quality cell culture reagents for researchers. Its culture reagents have been published in a lot publications that includes Nature an international leading scientific journal.

Culture Media

  • Animal origin-free
  • Sterility tested for bacteria, fungus and mycoplasma
  • Endotoxin tested
  • pH 7.1-7.5
Product nameProduct No.PKG Size
DMEM (1.0g/l Glucose) with L-Gln and Sodium Pyruvate, liquid08456-65500 ml
08456-3610 bottles x 500 ml
DMEM (1.0g/l Glucose) with Sodium Pyruvate, without L-Gln and Phenol Red, liquid08490-05500 ml
DMEM (4.5g/l Glucose) with L-Gln and HEPES, without Sodium Pyruvate, liquid08457-55500 ml
DMEM (4.5g/l Glucose) with L-Gln and Sodium Pyruvate, liquid08458-45500 ml
08458-1610 bottles x 500 ml
DMEM (4.5g/l Glucose) with L-Gln, without Sodium Pyruvate, liquid08459-35500 ml
08459-6410 bottles x 500 ml
DMEM (4.5g/l Glucose) without L-Gln, Sodium Pyruvate and Phenol Red, liquid08489-45500 ml
DMEM (4.5g/l Glucose) without L-Gln and Sodium Pyruvate, liquid08488-55500 ml
DMEM (4.5g/l Glucose) with Sodium Pyruvate, without L-Gln, liquid11584-85500 ml
DMEM (4.5g/l Glucose) with HEPES, without L-Gln and Sodium Pyruvate, liquid11585-75500 ml
DMEM (No Glucose) with L-Gln, without Sodium Pyruvate, liquid09891-25500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, liquid08460-95500 ml
DMEM/Ham's F-12 with L-Gln, Sodium Pyruvate and HEPES, without Phenol Red, liquid05177-15500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES, liquid11581-15500 ml
DMEM/Ham's F-12 with L-Gln and Sodium Pyruvate, without HEPES and Phenol Red, liquid11582-05500 ml
DMEM/Ham's F-12 with Sodium Pyruvate and HEPES, without L-Gln, liquid11583-95500 ml
DMEM/Ham's F-12 (No Glucose) with L-Gln and Sodium Pyruvate, liquid09893-05500 ml
IMDM with L-Gln and HEPES, liquid (Iscove's Modified Dulbecco's Medium)11506-05500 ml
Ham's F-12 with L-Gln, liquid17458-65500 ml
MEM with Earle's Salts and L-Gln, liquid21442-25500 ml
MEM with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid21443-15500 ml
MEM (No Glucose) with Earle's Salts, L-Gln and Non-Essential Amino Acids, liquid09848-05500 ml
α-MEM with L-Gln, Ribonucleosides and Deoxyribonucleosides, liquid21444-05500 ml
α-MEM with L-Gln, without Ribonucleosides and Deoxyribonucleosides, liquid21445-95500 ml
RPMI 1640 with L-Gln and HEPES, liquid30263-95500 ml
RPMI 1640 with L-Gln, liquid30264-85500 ml
30264-5610 bottles x 500 ml
RPMI 1640 with L-Gln, without Phenol Red, liquid06261-65500 ml
RPMI 1640 with L-Gln, liquid05176-25500 ml
RPMI 1640 (No Glucose) with L-Gln, liquid09892-15500 ml
 

Balanced Salines

Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin.

Product nameProduct No.PKG Size
D-PBS(+) Preparation Reagent (Ca,Mg Solution) (100x)02492-9430 ml
D-PBS(-) without Ca and Mg, liquid 14249-95500 ml
14249-24 10 bottles x 500 ml 
D-PBS(-) without Ca and Mg, liquid(10x)11482-15500 ml 
D-PBS(-) without Ca and Mg, Powder07269-84100 g
HBSS(+) with Ca, Mg and Phenol Red, liquid17459-55500 ml
HBSS(+) with Ca, Mg, without Phenol Red, liquid09735-75500 ml
HBSS(-) without Ca and Mg, with Phenol Red, liquid17460-15500 ml
HBSS(-) without Ca, Mg and Phenol Red, liquid17461-05500 ml

Supplements

Sterilized by 0.2 µm membrane filter, tested for bacteria, fungus, mycoplasma and endotoxin

Product nameProduct No.PKG Size
L-Alanyl-L-glutamine01102-8225 g
200mmol/l L‐Alanyl‐L‐glutamine Solution(100x)04260-64100 ml
200mM‐L‐Glutamine Stock Solution16948-04100 ml
1mol/l-HEPES Buffer Solution17557-94100 ml
MEM Non-Essential Amino Acids Solution(100x)06344-1420 ml
06344-56100 ml
100mM-Sodium Pyruvate Solution(100x)06977-34100 ml
apo-Transferrin from Human34401-84100 mg
34401-55500 mg
 

Cell Dissociation Reagents

Product nameProduct No.PKG Size
2.5g/l-Trypsin Solution35555-54100 ml
5.0g/l-Trypsin/5.3mmol/l-EDTA Solution35556-44100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution35554-64100 ml
2.5g/l-Trypsin/1mmol/l-EDTA Solution, 
with Phenol Red
32777-44100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution35553-74100 ml
0.5g/l-Trypsin/0.53mmol/l-EDTA Solution, 
with Phenol Red
32778-34100 ml
0.2g/l-EDTA Solution14367-74100 ml
 

Antibiotics

  • Animal origin-free
  • Sterility tested for bacteria, fungus and mycoplasma
  • Endotoxin tested
  • pH 7.1-7.5
Product nameApplicationProduct No.PKG Size
Actinomycin D Solution(1mg/ml)Other Antibiotics00393-411 ml
Antibiotic-Antimycotic Mixed 
Stock Solution(100x)
Bacteria, Fungal, Yeast02892-54100 ml
Antibiotic-Antimycotic Mixed 
Stock Solution(100x)(Stabilized)
Bacteria, Fungal, Yeast09366-44100 ml
Colcemid Solution(10µg/ml)Other Antibiotics09356-7410 ml
G 418 DisulfateSelection Antibiotics16512-36250 mg
16512-811 g
16512-945 g
16512-5225 g
G 418 DisulfateOther Antibiotics08973-011 g
08973-145 g
G 418 Disulfate Aqueous SolutionSelection Antibiotics09380-8620 ml
09380-44100 ml
Gentamicin SulfateBacteria/Mycoplasma08975-811 g
08975-945 g
Gentamicin Sulfate Solution (10mg/ml)Bacteria/Mycoplasma16672-0410 ml
Hygromycin B Selection Antibiotics 07296-66100 mg
07296-111 g
07296-24  5 g
Hygromycin B SolutionSelection Antibiotics09287-8420 ml
Kanamycin MonosulfateSelection Antibiotics08976-711 g
08976-845 g
Mitomycin C Solution(1mg/ml)Other Antibiotics20898-211 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
26253-84100 ml
Penicillin-Streptomycin-Glutamine Mixed Solution
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml, L-Glutamine 29.2mg/ml,
Sodium Chloride 0.14%, Citrate Buffer Solution 10mM
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
06168-34100 ml
Penicillin-Streptomycin Mixed Solution (Stabilized)
Penicillin 10,000u/ml, Streptomycin 10,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
09367-34100 ml
Penicillin-Streptomycin Mixed Solution
Penicillin 5,000u/ml, Streptomycin 5,000µg/ml
Bacteria(Gram-positive bacteria/
Gram-negative bacteria)
26252-94100 ml
Streptomycin SulfateGram-negative bacteria32204-345 g
32204-9225 g